Mass Spectrometry Detection of Histidine Phosphorylation on NM23-H1
نویسندگان
چکیده
منابع مشابه
Nm23-H1 metastasis suppressor phosphorylation of kinase suppressor of Ras via a histidine protein kinase pathway.
The metastasis-suppressive activity of Nm23-H1 was previously correlated with its in vitro histidine protein kinase activity, but physiological substrates have not been identified. We hypothesized that proteins that interact with histidine kinases throughout evolution may represent partners for Nm23-H1 and focused on the interaction of Arabidopsis "two-component" histidine kinase ERS with CTR1....
متن کاملHistidine to aspartate phosphotransferase activity of nm23 proteins: phosphorylation of aldolase C on Asp-319.
nm23 genes have been implicated in the suppression of tumour metastasis and cell motility; however, the biochemical mechanisms for these suppressions are not known. We have previously described the transfer of phosphate from the catalytic histidine residues of nm23 proteins to an aspartic or a glutamic residue on one or more 43 kDa proteins in detergent extracts of bovine brain membranes. To ga...
متن کاملSite-directed mutation of Nm23-H1. Mutations lacking motility suppressive capacity upon transfection are deficient in histidine-dependent protein phosphotransferase pathways in vitro.
We previously compared the structure and motility suppressive capacity of nm23-H1 by transfection of wild type and site-directed mutant forms into breast carcinoma cells. Wild type nm23-H1 and an nm23-H1(S44A) (serine 44 to alanine) mutant suppressed motility, whereas the nm23-H1(P96S), nm23-H1(S120G), and to a lesser extent, nm23-H1(S120A) mutant forms failed to do so. In the present study wil...
متن کاملAnalysis of protein phosphorylation using mass spectrometry.
Protein phosphorylation has been known to be a pivotal modification regulating many cellular activities and functions. Except for several conventional techniques, mass spectrometry-based strategies are increasingly considered as vital tools that can be utilized to characterize phosphorylated peptides or proteins. In this article, we summarized currently available mass spectrometry-based techniq...
متن کاملTwo-component kinase-like activity of nm23 correlates with its motility-suppressing activity.
Nm23 genes, which encode nucleoside diphosphate kinases, have been implicated in suppressing tumor metastasis. The motility of human breast carcinoma cells can be suppressed by transfection with wild-type nm23-H1, but not by transfections with two nm23-H1 mutants, nm23-H1(S12OG) and nm23-H1(P96S). Here we report that nm23-H1 can transfer a phosphate from its catalytic histidine to aspartate or ...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2011
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr100905m